Chih-Jen Lai

2 Questions 3 Answers 0 Followers

Questions related from Chih-Jen Lai

How to prevent mRNA degradation on gel electrophoresis?

I prepared DNA template (5kb) from PCR for in vitro transcription and purified the DNA by gel extraction and zymo column to avoid salt contamination (A260/230>1.8). However, I ran the...

24 July 2021 8,965 4 View

Low yield of mRNA after in vitro transcription?

I am currently working on in vitro transcription and got the problem with low mRNA yield. I prepared fresh IVT buffer and 2.5ug DNA template for 100ul Rxn and used N1-Methylpseudo-UTP, ATP, CTP,...

18 June 2021 7,521 3 View