Chien-Wei Chang

2 Questions 3 Answers 0 Followers

Questions related from Chien-Wei Chang

Does any one have experience with IF staining of the Golgi apparatus and associated transport vesicles in cardiomyocytes?

I've tested many conditions. Eventually, methanol fixation combined with permeabilization worked well in mouse N2A cells (e.g., COPII, see Figure 1). However, when I applied the same protocol to...

10 May 2025 6,107 3 View

Weird bubble-like spots on the Western blot membrane?

Hi, I have a question regarding western blot. I performed total protein statin after trasfering membrane. The result was totally fine before (Figure 1). However, there were strange bubble-like...

31 May 2024 8,150 3 View