Chiara Giacomelli

5 Questions 4 Answers 0 Followers

Questions related from Chiara Giacomelli

Anyone has experience with Aldehyde Dehydrogenase Activity Colorimetric Assay Kits?

Does anyone in the community have experience with Aldehyde Dehydrogenase Activity Colorimetric Assay Kits? (eg. http://www.sigmaaldrich.com/catalog/product/sigma/mak082?lang=de®ion=DE) Are...

04 April 2016 7,910 2 View

Any easy way to query the promoter of a gene for TF binding sites?

Is there any web tool to use where you can insert the gene ID of Your Favourite Gene, and the result is a list of putative/validated TF binding the promoter of YFG? Without having to search for...

04 April 2016 2,381 5 View

SiRNA control transfection on top of siRNA against target gene to equalize concentration in phenotypic assay?

Considering a phenotypic assay (proliferation/viability), would you transfect together with the target gene siRNA also negative ctrl siRNAs to achieve the same concentration as the multiple target...

10 October 2014 3,916 4 View

Has anyone ever used the miRcode for target prediction?

Has anyone used this http://www.mircode.org/mircode/index.php for target prediction? If so, any comment on how it performs? eg. compared to the usual TargetScan.

05 May 2013 5,810 0 View

microRNA overexpression at 25nM - can we assume effects on targets of miRNA of the same family?

In an miRNA overexpression experiment, say miR-17, with mimic transfected at 25 nM, is it possible that the effect is achieved through targeting of mRNA that are actually targets of miRNA from the...

03 March 2013 9,378 7 View