Chiara Mazzotti

3 Questions 10 Answers 0 Followers

Questions related from Chiara Mazzotti

Minimum FRET efficiency by acceptor photobleaching?

Hello, I am measuring heterodimerization of GPCRs by FRET acceptor photobleaching, using the CFP/YFP pair. I am using cells transfected only with GPCR-CFP as negative control, in order to measure...

26 June 2018 3,983 5 View

Why beta-arrestin-GFP does not translocate to the cell membrane after receptor activation?

I co-transfected HeLa cells with beta-arestin2-GFP and a 7TM receptor known to recruit arrestin upon ligand binding. I saw a strong expression of arrestin-GFP in my cells and I verified also the...

12 April 2016 6,807 12 View

Which is the best receptor-mediated endocytosis inhibitor?

I am studying the scavenging of a cytokine by its receptor, which I presume being internalized by clathrin-coated pits. I want to use the inhibitor to prove that the detected scavenging is due to...

04 September 2015 5,309 7 View