Chhaya Godse

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Questions related from Chhaya Godse

Can any body suggest method for acid urea gel electrophoresis for separation of proteins having marginal difference, in molecular weight, few Dalton?

Can any body suggest method for acid urea gel electrophoresis for separation of proteins having marginal difference in molecular weight --- few Dalton: In our experience i) Urea gel is very...

11 November 2016 9,874 5 View

Can anyone suggest a method to ensure uniform distribution of monocytes/macrophages before using them for activation assay with LPS or PMA?

I use GMCSF induced monocyte/macrophage culture (from PMBC) on 7th day for the activation assay, but always doubt the number of cells present per well. For 24 well plate I add 0.5 to 1x106 PBMC...

04 April 2013 3,754 3 View