Does anyone have experience with pCold TF DNA vector for expression of bacterial recombinant protein in soluble form?

I have cloned a cyanobacterial Aldo-Keto Reductase gene in pET/BL21 system and tried to express protein at lower temperature (16c) and IPTG concentration (0.05mM) to avoid inclusion body formation...

11 November 2014 8,526 6 View