Chetan Arya

2 Questions 7 Answers 0 Followers

Questions related from Chetan Arya

Expression of multi disulfide bonds containing protein in bacterial system?

I have a insect protein which contains 4-6 disulfide bonds in its active form. I want to use a bacterial expression system (or yeast if it does not work out for bacteria) for over-expression of a...

13 June 2019 5,033 5 View

What information/s can I get from the data in which I excited my protein at 383nm and got emission at 450nm. ?

My protein has color of brown but in UV_Vis spectroscopy no distinct band after 280nm. At high conc. of protein I can observe at shoulder at 383nm so I excited my protein sample at 383nm and took...

18 December 2014 2,624 10 View