Cheng Xu

3 Questions 2 Answers 0 Followers

Questions related from Cheng Xu

How can I do DNA FISH on a short region (~1kb) in the genome?

Hi! I'm new in the field of Fluorescent in Situ Hybridization (FISH). I want to use DNA FISH to visualize a small region in the human genome (around 1kb). I'm not sure if this is too short to use...

04 April 2019 9,096 3 View

What can be possible reasons for failure to express EGFP with MMTV-LTR promoter in mammalian cells?

I plan to use mouse mammary tumor virus-long terminal repeats (MMTV-LTR, it is a well-characterized mammalian promoter which can be induced by Dexamethasone) to express EGFP in MCF-7 breast cancer...

11 November 2016 2,143 1 View

Could anyone help me see if it is contamination in cell culture?

I'm a novice in mammalian cell culture. I'm culturing some MCF-7 cells (don't care about cells, they are not growing well because there's something wrong with the medium). I can frequently see...

04 April 2016 9,716 5 View