Cheng Dong

3 Questions 2 Answers 0 Followers

Questions related from Cheng Dong

In Electrophoretic Mobility Shift Assay (EMSA), after cold probe added(5×,20×,50× ) , free probe weaken in gradient?

In Electrophoretic Mobility Shift Assay (EMSA), after cold probe added(5×,20×,50×) , free probe weaken in gradient. The bound DNA had no obvious change. Anyone know why?

06 June 2019 2,804 1 View

How to predict regulatory element in promoter sequence in insect?

I have a promoter sequence of one gene in silkworm and how to predict the TSS sites or core promoter sites? Besides, is there any online website that can predict regulatory elements or TF biiding...

07 July 2018 4,614 0 View

Any suggestion can you provide if I do chip-seq from limited numbers of cells ?

Hello, everyone. Recently, I did chip-seq (H3K4me1、H3K4me3、H3K27ac、H3K27me3、Pol II、P300) from 1×107 insect cells. But, I got poor yield (about 4ng ) as standard Illumina library preparation needs...

05 May 2016 6,697 6 View