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We have been trying to flow sort live CD45+ cells from mouse intestines. One major issue is that these live cells continue dying after sorting. This is very critical for our following experiments...
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Recently we submitted a manuscript with some results of RNAseq. I used Gene Ontology and KEGG to do enrichment analysis for biological processes and pathways. Somehow one reviewer who might not be...
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My lab has done 16S and whole genome shotgun on mouse gut/fecal microbiome (wild-type vs knockout). Recently we found that the culturable part of these knockout mouse fecal samples are actually...
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My lab has been working on gut microbiota using mouse models for quite a few years and I read quite some gut microbiota papers using mono-association of germ-free mice with certain bacteria...
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