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I have worked on metabolomics data and I am not sure the principle of analysis. First is to conduct a PCA. Literature review says it is to test the overall stability of the system. I do not...
10 June 2021 4,807 2 View
At the last step for data analysis, we always use a STOP solution to stop the TMB oxidized by HRP. Why do we have to stop the reaction? Couldn't we measure the color wavelength of...
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I just want to know a question in terms of the result I got after PCR. The final PCR products contain my template DNA, amplified DNA, and dNTPs. How can I distinguish the parental DNA and...
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I am working on mutagenesis recently and I need to mutate glumamine to cysteine for the labeling reaction. According to their codons, I need to change three nucleotides. I prefer to put the...
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I am trying to express full-length tau with high yield and purity. I only used SEC to purify my protein since it is not his-tagged. When I did the UV-Vis to check the concentration of the tau...
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