Chen Zheng

3 Questions 6 Answers 0 Followers

Questions related from Chen Zheng

GST purification: No NaCl in Elution Buffer?

Hi I am trying to purify a complex, GST-tagged protein. This protein is prone to aggregate and unstable. My column was packed using Sepharose 4B. My question is : I found almost all protocols...

11 November 2016 5,719 4 View

Can anyone give suggestions about ShRNA selection within PC12 cells?

I'm trying to get a stable knockdown of my target gene using 3rd generation shRNA lenti-vector within undifferentiated PC12 cells. The shRNA lenti-vector has eGFP and puromycin selection feature....

03 March 2015 6,776 4 View

Does anyone know whether 3rd generation lentivector can be used with 2nd generation packing system & envelop plasmid?

I am trying to express shRNA to knockdown my target gene using lentiviral constructs. I found a 3rd generation lentivector from a bio company. But I don't want to buy their packaging kit. Just...

08 August 2014 8,656 6 View