Chen Wang

2 Questions 2 Answers 0 Followers

Questions related from Chen Wang

How to purify of GFP-fused proteins under native conditions?

I use gfp agarose from Chromotek to elute the protein while i can only get a denaturing protein. How to elute the protein from beads under native condition since our target protein is an enzyme...

02 November 2022 2,239 1 View

How to elute enzyme protein and maintain the enzyme activity in the elution process by GFP-trap agarose kit (chromotek)?

Dear all, I have some questions about the elution process of GFP-trap agarose. What's the best way to elute the protein and keep the enzyme activity at the same time since the eluted protein need...

01 November 2022 9,730 5 View