Why TOPO-D plasmids with insert are lost in the liquid LB? (being present in the colony)?

I amplified several PCR products and the bands were pretty good in size and unique. The PCR products were inserted into pENTR/D-TOPO, then they were transfromed into E. coli (TOP10 and DH5 alpha)....

10 February 2020 3,460 4 View