Chen Zhang

5 Questions 6 Answers 0 Followers

Questions related from Chen Zhang

Why I can't see immunostaining fluorescence after neuron transfected?

Hi all, I have a question about my lip3000 transfect neurons. I transfected my primary neurons with a control(mRFP) plasmid. Then I did immunostaining with primary proteinX antibody and Alexa 488...

16 March 2022 5,274 0 View

How can I check the RT-PCR product that are small(~90bp) on an agarose gel?

Hi everyone, I was trying to check the RT-PCR product which is less than 100bp on the agarose gel. I used 3% agarose and ran 80V for 1.5 hr, then I put my gel in the 1XTAE buffer with GelRED to...

10 December 2021 9,769 3 View

Can I use phrodo Red AM Intracellular pH Indicator to measure cytosolic pH?

Hello everyone. I want to measure intracellular pH. I want to co-stain cell with another Green indicator, can I use phrodo Red AM? I am not sure whether phrodo Red AM is sensitive enough to...

22 August 2019 7,534 1 View

Can I make pH 7.4 Sodium orthovanadate?

Hi everyone, I am trying to make 1mM sodium orthovanadate (Na3VO4) for the live-cell imaging experiment. According to the protocol, pH should be adjust to 10, however, can I reduce it to pH7.4...

28 April 2019 3,233 6 View

Can I do Multiple sites saturation mutagenesis?

Hello, I am trying to made saturation mutations at two different positions. I used QuickChange II mutagenesis kit and it is very powerful (the single saturation mutagenesis works) However, I added...

21 February 2019 7,081 4 View