What should be considered to improve western blotting of polypeptide at 4 to 7 kda size?

Hi all, I have been trying to detect several polypeptides by WB. And tricine-sds-page (16.5% or 20%) and glycine-sds page(20%) gel electrophoresis followed by Comassie blue staining showed no...

20 November 2023 7,384 3 View