Chee Hoo Yip

4 Questions 5 Answers 0 Followers

Questions related from Chee Hoo Yip

How can I determine the correct amount of plasmid and insert concentration when performing Gibson cloning?

Good day to all. I am encountering a problem in determining the correct amount of plasmid and inserts' concentration. According to the Gibson assembly protocol, I should include a 0.2-1 pmol of...

12 March 2015 1,344 3 View

Do I have to include a LacI repressor region when using an inducible T7 promoter?

Dear all, I am currently working on a synthetic biology project where I have to construct my own plasmid for protein expression. In regards with protein expression, I have chosen the inducible T7...

06 February 2015 6,977 3 View

Does anyone know what genes are mutated in Serratia marcescens WF mutant?

It is understood that S. marcescens WF mutant can only produce the MAP (monopyrrole moeity) but not the bipyrrole moeity (MBC). Hence, no condensation between these two moeities to form the final...

06 November 2014 8,822 0 View

How do I exclude internal promoters?

Dear all, I am now working with a bacterial gene cluster and located 26 internal promoters within this cluster. Most of these promoters are found on coding region and I plan to remove them for...

28 October 2014 494 8 View