Charumathi Pushparaj

3 Questions 12 Answers 0 Followers

Questions related from Charumathi Pushparaj

Has anyone seen that there is GRP78 upregulation in the mRNA level but not in the protein level, in spite of GADD153 upregulation and XBP1 splicing?

I work with ER stress in primary cultures of cardiomyocytes. I have checked induction of GRP78 and other ER stress markers by both realtime PCR and western blotting. In spite of induction of...

02 February 2014 5,846 12 View

Can someone advise on primary antibody in milk?

I use TBS1X- Tween 0.1% for using primary antibodies for western blot. I had problems with one protein and a friend suggested me to try in 5% skimmed milk with sodium azide. The antibody worked...

07 July 2013 6,707 6 View

What substrate to use to attach cardiomyocytes to glass coverslips?

I work with neonatal rat ventricular cardiomyocytes, and I want to do calcium imaging of the same. I have been trying to attach cardiomyocytes to glass coverslips and I have tried a few substrates...

03 March 2013 7,797 8 View