What should be the ideal settings for HSP length cut-off and HSP-Hit coverage for running Blast in Blast2Go?

I have to analyze PacBio transcriptome data with four bin sizes: 1-2 Kb, 2-3 Kb, 3-6 Kb and 5-10 Kb. I can understand that the HSP length cut-off and HSP-Hit coverage settings must be customized...

27 July 2017 1,472 1 View