Charlotte Mary Oakley

6 Questions 18 Answers 0 Followers

Questions related from Charlotte Mary Oakley

Does anyone have any advice on measuring SP-1 or SP-3 activation in vitro and in vivo?

Hi All, I'm trying to work out a way of measuring SP-1 and SP-3 activation in alveolar macrophages in vivo (I can perform a lavage to extract the cells first), and in bone marrow derived...

13 January 2016 1,433 0 View

Does anyone have any idea how to account for batch to batch variability in LPS potency?

Hi All, We always have a problem in the lab when starting a new batch of LPS as the potency seems to vary wildly. Apparently it's a very common problem - I was just wondering if anyone knew of a...

06 May 2015 3,327 3 View

Anyone know a good IL-1RA antibody for flow cytometry for murine cells?

Hi all, I'm trying to look at IL-1ra expression in lung injury but I'm struggling to find an antibody. Does anyone have any suggestions? Thanks

17 March 2014 6,304 2 View

What is the best way to measure tissue repair in the lungs?

I am looking at resolution after acute lung injury and am trying to ascertain what measurements are appropriate for looking at resolution.

24 February 2014 3,060 3 View

Does anyone have any experience with measuring AP-1 activation?

I'm looking to study AP-1 activation in some in vivo models of lung injury and was wondering whether anyone has any experience in doing this? Preferably I'd like to study it via flow cytometry but...

11 February 2014 2,736 3 View

Extraction of alveolar macrophages?

Does anyone have any experience with the recovery of alveolar macrophages? We are struggling to get a decent yield because as soon as we infuse saline or media the macrophages seem to adhere very...

05 November 2013 6,480 27 View