Charles Stewart Jr

5 Questions 5 Answers 0 Followers

Questions related from Charles Stewart Jr

Looking for suggestions for improving the order of protein crystals grown with Pentaerythritol propoxylate?

Is there anything about a protein that I can learn from the fact that it crystallizes in 35% Pentaerythritol propoxylate. I am working on crystallizing a protein and keep hitting dead ends. I...

10 October 2018 9,897 3 View

Advice for expressing chloroplast encoded protein in E.coli?

I want to express a protein which comes from plants in E.coli for assays and structural studies. I am ordering a synthetic version of the gene that is codon optimized for E.coli. When I run the...

01 January 2015 8,440 6 View

Any reasonable methods to measure the dipole moment of an alpha-helix in a protein?

I have a protein that contains an active site residue at the end of an alpha-helix. It has long been postulated that the dipole moment of this helix contributes to its activity. I want to compare...

02 February 2014 299 2 View

Does anyone have advice on measuring the decarboxylation of malonyl-CoA using using in vitro assays of recombinant proteins?

I have a protein that decarboxylates malonyl-CoA to form acetyl-CoA as part of it's catalytic activity. I want to directly measure the steady state kinetics for this decarboxylation step in the...

07 July 2013 8,206 4 View

What factors affect protein melting/unfolding curves for analyses by Thermofluor (i.e. differential scanning fluorimetry)?

I want to test ligand-binding on Tm of my protein. In pilot studies I can clearly detect the protein unfolding. However when I add the ligand (100 microM ligand to 1microM protein) I can not...

04 April 2013 10,055 45 View