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Questions related from Charles Karavina
I ran a gradient PCR and got multiple bands on all the temperature regimes that I had programmed. Why was this the case? How can I correct this so that I get the optimum temperature?
07 March 2015 4,411 5 View
I have been using gene specific primers for PCR. The primer synthesis report gave a lower Tm (by 7 degrees Celsius) than has been used elsewhere for the same primers. I have tried to optimize, but...
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I have been running gels on 1X TAE buffer. But I have noted that others have used 5X TAE buffer. What would be the advantages of the 5X TAE over the 1X TAE buffer?
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Has anyone sampled for RNA viruses in sub-tropical conditions using FTA cards?
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