Charles Karavina

4 Questions 12 Answers 0 Followers

Questions related from Charles Karavina

What causes multiple bands in gradient PCR?

I ran a gradient PCR and got multiple bands on all the temperature regimes that I had programmed. Why was this the case? How can I correct this so that I get the optimum temperature?

07 March 2015 4,403 5 View

How do I determine primer annealing temperature?

I have been using gene specific primers for PCR. The primer synthesis report gave a lower Tm (by 7 degrees Celsius) than has been used elsewhere for the same primers. I have tried to optimize, but...

25 February 2015 2,028 18 View

Why do we use 5X TAE buffer?

I have been running gels on 1X TAE buffer. But I have noted that others have used 5X TAE buffer. What would be the advantages of the 5X TAE over the 1X TAE buffer?

16 April 2014 3,977 1 View

How long can RNA be stored on FTA (Whatman) cards?

Has anyone sampled for RNA viruses in sub-tropical conditions using FTA cards?

04 November 2013 4,068 1 View