Charles Herr

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Questions related from Charles Herr

Anyone have experience getting fertilized zebrafish embryos to survive past epiboly/gastrulation (4 hours).

The fish were from Ekkwill Water-life Resources. They are referred to as the EKK strain. After four hours of normal looking development, cell blebbing occurs. Sperm are collected into Hank's...

05 February 2014 6,505 6 View

When making cell suspensions, why is EDTA added at the same time as trypsin, when trypsin is a metalloprotease that requires calcium?

For years we have made cell suspensions from sliced organs using the standard EDTA and trypsin method found in many lab protocols. I have always known that many proteases are metalloproteins. I...

01 January 1970 4,729 4 View