Can I purify a protein with N-term ORF-histx6-HA-ProteinA(ZZ)-Cterm on an hist-trap(Ni affinity column)? Is it only possible if hist tag is terminal?

If during cloning my construct is N-term ORF-histx6-HA-ProteinA(ZZ)-Cterm, can I still purify the protein on hist-trap column. Will the 6-hist be still accessible to attach hist-trap column. NOTE...

04 April 2015 9,371 7 View