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I used the following procedure to transform DH10Bac E. coli. I wanted to know why my experiment failed. Exp. Procedure: 1. Thaw 25ul DH10Bac competent cells on the ice for each transformation. 2....
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I purified the virus(H1N1/PR8) protein from the allantoic fluid by 20%-50% sucrose gradient, and I used the SDS-PAGE to separate the HA protein; I loaded 5ug protein per well; after coomassie blue...
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