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My gene is cloned in pET28a+ and in then transformed into BL21 DE3. My gene size is about 70 kDa. I have used 1 % glucose during induction and using Terrific broth as a medium. Initially also i...
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I am trying to express mammalian protein in bacterial system. Protein Size 70kDa, vector backbone pET28a+. I received the gene in pUC backbone, when transformed in DH5a, showed clonal variation....
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