Chandrakala Gowda

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Questions related from Chandrakala Gowda

Unable to redissolve protein from the ammonium sulphate precipitate. Any suggestions?

I performed protein precipitation using 40% ammonium sulphate precipitation as follows 1. 5.5 grams of ammonium sulphate salt added very slowly over a 30minutes time with gentle stirring for a...

19 June 2017 6,113 10 View

I have too much noise in the aliphatic region of 13C NOESY spectrum. Any inputs about how it could be minimised?

~ 13kDa protein, 100ms mixing, 1 second recycle delay, Bruker Pulse Seqeuence used is noesyhsqcetgpsi3d. Noise appears parallel to the F1(H) in the F1-F3 plane. where F1 and F3 correspond to...

20 January 2016 9,290 4 View

Cell clumps/floating debris found in primary M9 overnight culture?

I inoculate my 100ml M9 culture with cells from a 10ml MDAG - 135 media of 8 hours growth. After overnight incubation at 37degrees I see a good growth of the cells upto OD 2.0 but with also large...

01 January 1970 5,030 3 View

Very slow cell growth in M9 media with a generation time of roughly 4hours?

I have a situation with bacterial growth in M9 media with two plasmidsWild Type (WT) and Mutant. BL21DE3-PLysS cells. procedure followed is as follows 1. Incoulated 5ml LB primary with a single...

01 January 1970 5,961 5 View