Chandrakala Gowda

8 Questions 22 Answers 0 Followers

Questions related from Chandrakala Gowda

Unable to redissolve protein from the ammonium sulphate precipitate. Any suggestions?

I performed protein precipitation using 40% ammonium sulphate precipitation as follows 1. 5.5 grams of ammonium sulphate salt added very slowly over a 30minutes time with gentle stirring for a...

19 June 2017 6,149 10 View

BMRB submission of pdb structure obtained from cyana ?

My question is basically for someone who is familiar with CYANA software and have successfully submitted a pdb file calculated using NMR restraints using CYANA I am trying to execute the macro...

17 May 2017 9,759 0 View

Cyana structure calculation analysis of final.ovw?

Need some help in trouble shooting cyana structure calculation result ==== The following is from final.ovw Constraints violated in 6 or more structures: # mean max. 1 5 10 15 ...

18 December 2016 9,892 2 View

Is it possible to predict a secondary structure of a protein using only amide Nitrogen and Proton chemical shifts?

I have a HN HSQC spectrum from which I have been able to assign the chemical shifts of all the residues by comparing to literature spectra of similar samples. (its a small protein of size 52...

26 November 2016 5,934 11 View

Is it possible to assign a noesy spectrum with sparky/nmrfam. If not what other softwares are good for a reliable noesy spectra assignment.

17 February 2016 5,508 1 View

I have too much noise in the aliphatic region of 13C NOESY spectrum. Any inputs about how it could be minimised?

~ 13kDa protein, 100ms mixing, 1 second recycle delay, Bruker Pulse Seqeuence used is noesyhsqcetgpsi3d. Noise appears parallel to the F1(H) in the F1-F3 plane. where F1 and F3 correspond to...

20 January 2016 9,324 4 View

Cell clumps/floating debris found in primary M9 overnight culture?

I inoculate my 100ml M9 culture with cells from a 10ml MDAG - 135 media of 8 hours growth. After overnight incubation at 37degrees I see a good growth of the cells upto OD 2.0 but with also large...

01 January 1970 5,082 3 View

Very slow cell growth in M9 media with a generation time of roughly 4hours?

I have a situation with bacterial growth in M9 media with two plasmidsWild Type (WT) and Mutant. BL21DE3-PLysS cells. procedure followed is as follows 1. Incoulated 5ml LB primary with a single...

01 January 1970 6,009 5 View