Chandni Sood

3 Questions 12 Answers 0 Followers

Questions related from Chandni Sood

How to do transfection in THP1 cell line?

I want to transfect plasmid DNA in THP1 and have tried various methods but it's not working as cell death is too high.

02 February 2017 5,498 5 View

What should be done when a protein is getting precipitated during dialysis?

I purified my recombinant His tag protein in NaHPO4-50mM, Nacl-300mM,immidazole-20mM,bmercaptoethanol-10mM, glycerol-10% with pH 8 (pI of protein 9.45) and eluted in 500mM Imidazole conc. in...

07 July 2014 6,337 18 View

Does knocking out a gene can affect transcription of adjacent gene in bacteria?

I am trying to knockout a bacterial gene by homologous recombination. I have designed a knockout construct having 500 upstream, 500 downstream, 100 bp of each 5` and 3` end of the gene with a...

06 June 2014 2,516 12 View