3 Questions 13 Answers 0 Followers
Questions related from Challagandla Anil
Does anyone have a detailed protocol for PEG mediated plasmid DNA transformation? Which MW should PEG have for a plasmid of 8.6kb? Thank you in advance.
10 February 2015 3,526 3 View
Please can anyone suggest, weather DMSO is added (as a cryoprotectant) or not, to fungal protoplast. Since DMSO is a polar aprotic solvent I am scared of getting the protoplast membrane dissolved....
07 February 2015 7,811 8 View
Can any one suggest which method is the best for making E coli DH5a competent cells. Now I am using Inoue and rubidium chloride methods.
31 October 2014 494 8 View
