Céline S. Gonçalves

2 Questions 2 Answers 0 Followers

Questions related from Céline S. Gonçalves

Can I use Sybr Green (for the housekeeping gene) and Taqman assay (for the gene of interest)?

I’ve already validated the use of a good housekeeping gene in my samples with Sybr Green. However, for one of my genes of interest, I need to use Taqman assay. So, I’m wondering if I can use...

06 August 2013 2,531 13 View

Human cancer cell line culture contamination

I've experienced some problems culturing human cancer cell lines cultures and I can't really be sure if it is some kind of contamination. My main concern is that I observe black "dots/spots" in...

13 November 2012 7,833 76 View