Celia Gracia

4 Questions 7 Answers 0 Followers

Questions related from Celia Gracia

Does the WCLB (White Cell Lysis Buffer) for DNA extraction need to be autoclaved?

I´m using a Salting Out protocol for DNA extraction from blood.

31 August 2015 2,609 2 View

How can I get rid of the faint smear in PCR?

Hi all, My PCR products always show a very faint smear spreading all the way long (from the wells to the band of interest). I´m not being able to get rid of it. I have the same problem with two...

31 March 2015 8,635 17 View

What annealing T range to set in Touchdown PCR?

Hi, I'm trying to amplify a DNA fragment using primers with Tm: 79.3℃ and 63.4℃. I have tried T gradients between 54-68℃. There's always a loylt of unspecific bands. Ithink that a touchdown PCR...

29 January 2015 8,100 3 View

Is anyone familiar with PCR reaction reproducibility?

Hi, I am trying to find the right PCR conditions for the amplification of a 190bp DNA fragment. When using the same PCR mastermix, the PCR reaction gives very good specific yield for some of the...

14 January 2015 1,444 9 View