Cédric Chaveroux

5 Questions 15 Answers 0 Followers

Questions related from Cédric Chaveroux

Stemness features in cells grown on soft agar ?

Hi, I saw that growing cells on ultra low adherence plates is similar to soft agar assays. However, if i'm not wrong, making spheroids on these plates requires stem-like cells whereas soft agar...

11 November 2018 1,231 0 View

Recommandations for PI cell cycle analysis in 6-well plates ?

Hi everyone, We are trying to optimize a protocole for cell cycle analysis in 6-well plates. We prefer this format of plates because it is what we use regularly for siRNA transfections. So we...

11 November 2017 7,447 7 View

Reference genes for human tumors vs healthy tissue in qPCR ?

Hi Is there anyone who has a reliable gene we could use as a housekeeping gene in cancerous tissues? We have tried HPRT, Actin, 18S, ...they all move in the tumors (upregulated). Thank you

10 October 2016 8,420 5 View

CpG free promoter for generation of stable cell line ?

Hi, I would like to generate a cell line stably overexpressing a gene of interest. We normally use a system containing a CMV promoter. However I wonder if methylation of the promoter could occur...

02 February 2016 2,044 8 View

What are the precise sequences for Age1 site to add to oligos for shRNA cloning in pLKO.1?

Hi everyone, I'm planning to do shRNA cloning in the pLKO.1 for lentivirus production. i read the protocol provided by addgene et some others. There is a little that I don't understand regarding...

03 March 2015 7,053 5 View