Cécile Dubois

2 Questions 4 Answers 0 Followers

Questions related from Cécile Dubois

Has anybody ever seen these "waves" on a native gel ?

I'm working on proteasomal activity on C.elegans. I extracted worms with lysis buffer containing :20mM tris, 5mM MgCl2, 1mM DTT, 1mM ATP, 10% glycerol, 0,05 NP40. I ran a native gel...

24 November 2016 9,219 4 View

Does anyone have experience in native gel electrophoresis to measure proteasomal activity in C.elegans?

I performed a native gel electrophoresis after an extraction in native lysis buffer on C.elegans. But something is "stuck" in the gel (where the 26s 1 cap should be seen).I have used a 4%...

18 November 2015 9,266 2 View