Catherine Wright

3 Questions 5 Answers 0 Followers

Questions related from Catherine Wright

Can RFP in protein lysate interfere with BCA assay?

I transfected HEK293 cells with constructs containing an RFP reporter. The signal was so strong that the RFP was visible to the naked eye when I pelleted the cells. And the protein lysate even had...

10 October 2016 6,668 5 View

Does anyone have any advice for generating a plasmid map?

I have a construct labeled "Venus-tubulin" that is Amp resistant. There is no record in the lab about where the plasmid came from or how it was generated or who gave it to the lab. I have no...

02 February 2016 4,987 7 View

My Western blot/SDS-PAGE 10% gel ruffles at the corners. Can anyone tell me why?

I made a standard SDS-PAGE gel (5% stacking, 10% resolving) using the same recipe I have used successfully for years. I wrapped the gel in wet paper town and stored it at 4C for about 18 hours...

01 January 2016 268 6 View