Catherine Moroski

3 Questions 6 Answers 0 Followers

Questions related from Catherine Moroski

THLE-3 cell culture -- does the collagen source matter?

We recently ordered THLE-3 cells from ATCC. The flasks need to be coated with fibronectin, bovine serum albumin and collagen. ATCC uses bovine collagen type I. We have collagen type I isolated...

06 June 2014 9,141 1 View

Can I transfer cells to liquid nitrogen after 6-8 months of storage at -80C?

We recently purchased a liquid nitrogen storage freezer. Up until now, we have stored all our cells at -80C. I have stocks that have been stored at -80C for 6-8 months. Does anyone know if the...

05 May 2014 2,112 4 View

Why is the empty vector killing our cells?!

We are ectopically expressing splice variants of ING1 (a tumor suppressor) in some head and neck cancer cell lines using pcDNA3.1/His B plasmid. For some reason, the cells receiving empty vector...

12 December 2013 555 37 View