Cathal Dold

3 Questions 2 Answers 0 Followers

Questions related from Cathal Dold

QPCR - High biological variation in Ct values for reference and target gene with in vivo intestinal tissue extracted from pig?

I am currently performing qPCR work on intestinal (duodenum and jejunum) tissue collected from the pig small intestine and stored at -20°C in RNALater Stabilization Solution until qPCR analysis....

13 December 2021 565 3 View

QPCR - High variation between biological replicates in reference and target genes?

After performing qPCR on intestinal tissue collected from animals, I have high variability in Ct (difference of 5-7 Ct cycles) values within treatment groups (between biological replicates - 10...

26 November 2021 1,006 3 View

QPCR - NO-RT control amplification with only some primers and not other primers?

When carrying out cDNA synthesis using the SensiFAST™ cDNA Synthesis Kit, I prepared a NO-RT control by including water instead of reverse transcriptase (along with RNA up to 1μg and 5X TransAmp...

29 October 2021 2,776 3 View