Catarina A. Marques

6 Questions 39 Answers 0 Followers

Questions related from Catarina A. Marques

What is the correct way to represent overlayed FACS data when the number of acquired cells is different between samples?

Hello, I have data from an experiment in which I'm comparing cell cycle profiles (PI staining) in treated vs untreated cells. The problem was that in certain cases, the number of treated cells was...

07 July 2015 4,377 8 View

Can anyone help with YFP constructs to endogenously tag Leishmania proteins?

I'm looking to tag endogenously some proteins in Leishmania major, and since I'm trying to repeat something done before in T. brucei using the YFP tag, I was planning to do the same in L. major....

03 March 2014 9,303 2 View

FACS of Trypanosoma brucei parasites?

I am interested in sort blood stream forms (BSFs) of Trypanosoma brucei. So far I've been successful sorting the procyclic form cells of this parasite using a protocol that has been published...

12 December 2013 9,388 14 View

Any advice on imaging of nuclear proteins?

I've been having problems imaging nuclear proteins tagged with myc in Trypanosoma brucei. I've been using 4% PFA for fixation and 0.2% Triton X-100 for permeabilization. Although I can detect the...

12 December 2013 7,460 18 View

What is the minimal amount of DNA that can be used for a qPCR reaction?

I need to do some qPCR on qDNA samples that are at very low concentrations, such as 0.5-1 ng/ul. I have very few samples left, and I was wondering if anyone knows the minimum amount of DNA that...

11 November 2013 7,846 18 View

Is there a conserved sequence that defines an arginine finger motif in AAA ATPases?

I'm trying to check if the proteins I'm working on possess an arginine finger or not. The problem is that I've been having problems finding the sequence online (including papers). Most of the time...

09 September 2013 6,217 4 View