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Questions related from Catarina A. Marques
Hello, I have data from an experiment in which I'm comparing cell cycle profiles (PI staining) in treated vs untreated cells. The problem was that in certain cases, the number of treated cells was...
07 July 2015 4,393 8 View
I'm looking to tag endogenously some proteins in Leishmania major, and since I'm trying to repeat something done before in T. brucei using the YFP tag, I was planning to do the same in L. major....
03 March 2014 9,316 2 View
I am interested in sort blood stream forms (BSFs) of Trypanosoma brucei. So far I've been successful sorting the procyclic form cells of this parasite using a protocol that has been published...
12 December 2013 9,399 14 View
I've been having problems imaging nuclear proteins tagged with myc in Trypanosoma brucei. I've been using 4% PFA for fixation and 0.2% Triton X-100 for permeabilization. Although I can detect the...
12 December 2013 7,471 18 View
I need to do some qPCR on qDNA samples that are at very low concentrations, such as 0.5-1 ng/ul. I have very few samples left, and I was wondering if anyone knows the minimum amount of DNA that...
11 November 2013 7,856 18 View
I'm trying to check if the proteins I'm working on possess an arginine finger or not. The problem is that I've been having problems finding the sequence online (including papers). Most of the time...
09 September 2013 6,229 4 View