If using Q5 for mutagenesis, and complimentary primers that contain a 1-2bp substitution, is more than DpnI digestion required before transforming?

We recently had a tech that passed through who ordered Q5 for her mutagenesis PCRs. She clearly had things working because she created 10-20 constructs that I checked the sequences for. However,...

11 November 2019 6,423 3 View