Casey Vickstrom

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Questions related from Casey Vickstrom

Tips on GCaMP imaging in acute slices using AAV to express

For anybody who has done GCaMP imaging in acute brain slices, I have some questions. We injected AAV expressing GCaMP6f into a particular brain region, waited >2-3 weeks, cut slices, and are...

13 June 2017 3,862 1 View

Do AAVs tranduce axons passing through a region of interest?

If you are doing an experiment where you inject AAV into a specific region, e.g. AAV-DIO-ChR2-eYFP in a Cre driver line for optogenetics, will the AAV transduce the axons passing through the...

29 September 2016 239 2 View

Does stimulating severed axons in brain slice cause neurotransmitter release from those axons?

When you cut a slice, you sever many axons. So when you use your stimulating electrode (or a light in optogenetics) to evoke currents in the cell you've patched, can you evoke a response if the...

26 August 2016 4,721 3 View

How exactly does current and voltage clamping work?

When patching a cell, what exactly is the electrode doing that "injects" current into the cell? If there's no literal injection of charge/current into the cell, how can the cell do things like...

28 August 2014 7,145 9 View

What happens with constant current injection of neurons in current clamp?

With constant current pulses (lasting several hundred milliseconds), do neurons fire trains of action potentials or just one single action potential? Since voltage-gated Na+ channels can only...

28 August 2014 8,117 4 View