How can I collect the primary rabbit tracheal epithelial cells ?

I tried to get the epithelial cells from the tracheal mucosa. I incubated the tissues in S-MEM with 0.1% protease ⅩⅣ and 0.01%DNase at 4 ℃ overnight. But on the next day, I can only collect little...

01 January 2015 2,095 4 View