Caroline Pule

3 Questions 3 Answers 0 Followers

Questions related from Caroline Pule

Any advise on the ideal dilution factor to use for culture supernatants sample preparation for multiplex assay for cytokines stimulation?

Any advise on the ideal dilution factor to use for culture supernatants sample preparation for multiplex assay? I'm planning to run a multiplex assay experiment for the detection of...

12 May 2017 3,659 1 View

Can one use 0.1%Triton X-100 to lyse RAW macrophages infected with M. tuberculosis, if planning to extract RNA from the intracellular bacteria lysate?

Trying to find an effect alternative lysis buffer not that different to GITC that can ensure effective lysis of infected macrophages with Mycobacterium tuberculosis. If planning to extract RNA...

06 October 2016 371 4 View

Between 0.5 ug of RNA and 1 ug of RNA, which one will give good RT reaction in 20 ul reaction?

If I do cDNA synthesis with QunatiNova RT kit using 0.5 ug of total RNA in 20 ul RT reaction, would this give better result compared to if I 1 ug of total RNA for the same 20 ul RT reaction? My...

17 March 2016 5,529 1 View