Caroline Keroack

4 Questions 10 Answers 0 Followers

Questions related from Caroline Keroack

Any ideas (other than MITObim) for assembling mitochondrial genomes from full genomic illumina reads?

Hi! I am asking because I had some luck using the SPAdes assembler to assemble the mitochondrial genome from subsamples of full genomic reads of parasitic nematodes, but it has not been successful...

20 March 2016 5,195 10 View

Any suggestions for a program/pipeline to assemble mitochondrial genomes?

Hi, I have recently sequenced mitochondrial genomes of parasitic nematodes that were amplified by long range PCR. I am looking for suggestions for the best program to assemble these reads (paired...

27 November 2015 2,196 2 View

Can anyone help with the designing of primers to amplify entire mitochondrial genome from parasitic nematode?

I have been following the protocol from Hu, et al 2007 (nature protocols). Using their 40R and 5F and 42F 39R primers I have managed to amplify complete mitochondrial genomes from two strongylid...

07 August 2015 1,610 1 View

Can any one suggest a good method for isolating clean mitochondrial DNA from large (parasitic) nematodes?

I am trying to isolate mtDNA for next generation sequencing (I have worked on long PCR for 2 years and have decided to take this more direct approach). I have tried bench-top differential...

09 June 2015 4,134 8 View