Caroline Jackson

3 Questions 4 Answers 0 Followers

Questions related from Caroline Jackson

Why would there be multiple bands in free probe lane of EMSA?

I've been trying to obtain a gel shift, but my free probe (biotin-labeled probe run in a lane by itself) always yields multiple bands. I generated the probe by ordering 5' biotinylated primers for...

24 March 2016 2,279 3 View

Minimum protein size for gel shift assay?

Hi all, I was wondering if there was a minimum kDa value that a protein would need to have in order to cause a visible gel shift in an EMSA assay. My protein of interest is rather small (15-20...

15 March 2016 685 4 View

Why is my SDS-PAGE not running straight; wells are expanding/compressing?

Hi all, Recently I've been having issues with my SDS-PAGE gels not running properly. The samples don't run in a straight line as they should, but instead the lanes in the middle of the gel migrate...

10 March 2016 890 11 View