Carolina Md

3 Questions 1 Answers 0 Followers

Questions related from Carolina Md

Splicing by overlap extension PCR? How does the fragments join?

I'm using a protocol in which I digest a antibiotic resistance gene with KpnI and BamHI and it leaves overhangs on the same strand 3'-5'. Then I make two fragments A e B as showed in the picture...

22 November 2022 3,335 0 View

Bacterial transformation contamination?

I'm doing bacterial transformation on Helicobacter pylori first on non-selective medium and then in mueller hinton medium with the antibiotic. I always make a suspension of the biomass with BHI...

13 June 2022 3,623 2 View

Optical density (OD) in a bacterial culture?

I'm trying to extract plasmid DNA of e.coli and I'm growing a pre-culture in LB agitated medium at 37ºC. I should have an OD(600 nm) 2,6 to start another culture but it only reaches OD 1,16 and...

22 April 2022 5,391 3 View