Carolin Göbel

4 Questions 5 Answers 0 Followers

Questions related from Carolin Göbel

How to compare expected and counted values statistically with different p values for each genotype?

Hey there, I am currently working on the statistics of counted and expected genotypes in mice litter of a mutant mouse model. I have two different mutated genes, both alone and in combination, one...

04 February 2021 6,677 2 View

Can a mixed background in mice reduce cre recombinase activity in comparison to a pure Bl6 background?

Our transgenic mice show a really strong phenotype which differs a lot from results of another group working with the same transgene. As our mice are on a pure C57Bl6J background and theirs was on...

25 September 2020 2,005 3 View

How to increase efficiency of lentiviral transduction in primary neural cells?

I am trying to transduce primary murine cerebellar granule cells using a lentiviral system. I tried transduction with polybrene, but it seems to be quite toxic for my cells. However, transduction...

11 November 2019 8,657 6 View

How much digested plasmid DNA can be loaded onto an agarose gel for subsequent gel extraction?

I'm digesting a 11 kbp plasmid, resulting in three fragments (should be around 5, 4 and 2 kbp, respectively). What amount of digested DNA (in ng) would you recommend to be loaded onto a 1% agarose...

29 April 2019 571 3 View