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My PCR products are probably specific (e.g. only 1 band), so usually I use a PCR clean up kit to purify my PCR sample (I only check 2 ul of the PCR product with loading dye on an agarose gel)....
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In some protocols I see the addition of Tween to the HIER buffer (citrate or EDTA). My first question is, why is this, what is the mechanism of action? And my second question is then, what is...
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To isolate DNA from mouse ear pieces for genotyping, we use a digestion buffer and we add prot K. Our DNA digestion buffer contains: Tris-HCl (pH 8.0), EDTA (pH 8.0), NaCl and SDS. We always add...
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