Carmen Santana-Calvo

2 Questions 4 Answers 0 Followers

Questions related from Carmen Santana-Calvo

Does anybody have experience with expressing proteins in the surface of mammalian cells (i.e CHO or HEK cells)?

Hi. I'm using the pDisplay vector from Invitrogen to express in the membrane surface a cytosolic domain of a protein. The problem I have is that the protein is not displaying in the surface, I...

04 May 2015 9,797 3 View

What is the best buffer to obtain a circular dichroism spectrum of a protein?

I need to dialyze my protein to remove NaCl (300 mM) and imidazole (the current buffer also has 10 mM Tris HCl, pH 7.4). I want to exchange the protein to a buffer that only contain 10 mM Tris...

20 February 2015 9,569 6 View