Carlton Ranjith Wilson Alphonse

3 Questions 4 Answers 0 Followers

Questions related from Carlton Ranjith Wilson Alphonse

How to derive Km, Ki enzyme kinetics for beta lactamase and nitocefin?

I went through lot of literature and found there is some complication with calculating enzyme kinetics of beta lactamase, nitrocefin and an inhibitor. i am attaching a paper in this. please help...

15 April 2016 2,716 6 View

My solution has some moving objects when I add my inhibitor to buffer solution, will this affect the OD value?

During acetylcholinesterase assay I used a inhibitor for finding its inhibitory activity, during dissolving the compound in buffer the solution becomes like there is some dust in there with it. I...

21 June 2014 1,910 2 View

Is there any separate protocol to isolate mitochondria from zebrafish brain cells?

I need to isolate mitochondria and isolate its protein. Is there any separate protocol for that, or can I use the general protocol? Is there any procedure for immunohistochemisty of mitochondrial...

01 June 2014 3,516 10 View