Carlos Córdova-Fletes

3 Questions 15 Answers 0 Followers

Questions related from Carlos Córdova-Fletes

Can RNA isolation be effective from 1-day whole blood at room temperature WITHOUT stabilization solution?

Hi everyone, I will run a microarray assay using total RNA from 1-day whole blood (in EDTA) at room temperature but without stabilization solutions...Is it effective for microarray assays?? The...

03 March 2015 781 7 View

Is it advisable to average various qPCR normalization genes?

Hi everyone, is it advisable averaging of some endogenous genes for qPCR data normalizing?? Or should I use them by separately?? Thanks in advance, Carlos.

12 December 2014 5,244 9 View

Should I use the Agilent (low input) one-color or two-color protocol?

I will work on comparison of expression profiles from two different lymphoblastoid cell lines with particular disrupted genes in order to explore affected pathways. The array is Human GE 4x44K v2...

04 April 2014 635 4 View