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Hello everyone. I am struggling to get good quality results on my Sanger sequencing reactions. I don't know the specific problem that I am facing. In a single order, most of the chromatograms...
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Hello everyone. We are trying to design a multiplex qPCR to detect three different viral sequences, by using three pairs of primers and three different fluorescent quenched probes: 1) HEX -...
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Hello everyone. It is common knowledge that the chromatograms come cleaner if the PCR products are purified. However, I know some colleagues that send the amplicons to sequence without any...
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